Production of homogeneous glycoprotein with multisite modifications by an engineered N-glycosyltransferase mutant
نویسندگان
چکیده
منابع مشابه
Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins.
Ansamitocins are potent antitumor maytansinoids produced by Actinosynnema pretiosum. Their biosynthesis involves the initial assembly of a macrolactam polyketide, followed by a series of postpolyketide synthase (PKS) modifications. Three ansamitocin glycosides were isolated from A. pretiosum and fully characterized structurally as novel ansamitocin derivatives, carrying a beta-D-glucosyl group ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2017
ISSN: 0021-9258
DOI: 10.1074/jbc.m117.777383